Estrogen receptor ligand binding assay

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Song, L. Hou, T. Li, T. Guan, T. Zhang and Y. Wang, Anal. Search articles by author Jie Zhang.

Conservation of estrogen receptor function in invertebrate reproduction

Wenfu Wu. Yifan Song. Ligang Hou. Tiezhu Li. Tianzhu Guan.

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Kinetic analysis of estrogen receptor/ligand interactions

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Fluorescence response profiling for small molecule sensors utilizing the green fluorescent protein chromophore and its derivatives. ACS Comb Sci. Methods Mol Biol. Methods Enzymol. Chemical complementation: small-molecule-based genetic selection in yeast. Creation and discovery of ligand-receptor pairs for transcriptional control with small molecules. BAPJA: a yeast two-hybrid strain for both positive and negative genetic selection. J Microbiol Methods. Download references. We would like to thank the three anonymous reviewers and the editor Dr. Becks for very helpful suggestions on our paper.

Estrogen Receptor (ER) Binding Assays Using the CERI Human Recombinant ERα

We also thank Lelani Barry and Samantha Dean for their technical assistance. BLJ carried out rotifer culturing, genetic selection assays, homology modeling, completed the lifespan and reproductive assays and drafted the manuscript. CLW synthesized the fluorescent synthetic ligands and completed the docking simulations. All authors read and approved the final manuscript.

Correspondence to Brande L. Conserved amino acids are green. Similar amino acids are cyan. Amino acids that directly interact with the estradiol ligand are boxed rotifer, red; human; blue. These amino acids are highly conserved as well. XLS kb. These compounds were chosen because previous work verified their binding to the human ER LBD in yeast chemical complementation assays.

These compounds fluoresce upon binding to their target [ 35 ]. Estradiol is the natural ligand for the human ER and does not fluoresce.