Estrogen receptor ligand binding assay
Try again? Cited by. Back to tab navigation Download options Please wait Article type: Paper. DOI: Download Citation: Anal. Zhang, W. Wu, Y.
Song, L. Hou, T. Li, T. Guan, T. Zhang and Y. Wang, Anal. Search articles by author Jie Zhang.
Conservation of estrogen receptor function in invertebrate reproduction
Wenfu Wu. Yifan Song. Ligang Hou. Tiezhu Li. Tianzhu Guan.
Tiehua Zhang. Molecular evolution of the membrane associated progesterone receptor in the brachionus plicatilis rotifera, monogononta species complex. Effect of progesterone on sexual reproduction of Brachionus manjavacas Rotifera. J Exp Mar Biol Ecol. Discovering genes associated with dormancy in the monogonont rotifer Brachionus plicatilis. BMC Genomics. Analysis of expressed sequence tags of the cyclically parthenogenetic rotifer Brachionus plicatilis. PLoS One.
Kinetic analysis of estrogen receptor/ligand interactions
A review of the molecular mechanisms of monogonont rotifer reproduction. Effect of juvenile hormone and serotonin 5-HT on mixis induction of the rotifer Brachionus plicatilis Muller. Effect of growth hormone and gamma-aminobutyric acid on Brachionus plicatilis Rotifera reproduction at low food or high ammonia levels. Effect of some vertebrate and invertebrate hormones on the population growth, mictic female production, and body size of the marine rotifer Brachionus plicatilis Muller. Use of the freshwater rotifer Brachionus calyciflorus in a screening assay for potential endocrine disruptors.
Environ Toxicol Chem. Effects of progesterone, testosterone, and estrogen on sexual reproduction of the rotifer brachionus calyciflorus. Int Rev Hydrobiol.
Workshop on rotifers in ecotoxicology. Fluorescence imaging using synthetic GFP chromophores. Curr Opin Chem Biol. Aromatase, the enzyme responsible for estrogen biosynthesis, is expressed by human and rat glioblastomas. Neurosci Lett.
Molecular cloning of equine 17 beta-hydroxysteroid dehydrogenase type 1 and its downregulation during follicular luteinization in vivo. ERBP, a novel estrogen receptor binding protein enhancing the activity of estrogen receptor. Biochem Biophys Res Commun. Sievers F, Higgins DG. Clustal omega, accurate alignment of very large numbers of sequences. Mult Seq Alignment Methods. Creation and discovery of ligand—receptor pairs for transcriptional control with small molecules. Chemical complementation: a high throughput system for nuclear receptor drug discovery. Abstr Pap Am Chem Soc. Azizi B, Doyle DF.
Chemical complementation: a genetic selection system in yeast for drug discovery, protein and enzyme engineering. Chemical complementation: protein engineering through genetic selection. Duraj-Thatte A. Fluorescent GFP chromophores as potential ligands for various nuclear receptors. PhD diss. Vestigialization of an allosteric switch: genetic and structural mechanisms for the evolution of constitutive activity in a steroid hormone receptor.
Plos Genet. Independent elaboration of steroid hormone signaling pathways in metazoans.
- Estrogen Receptor Structures & Functions.
- Radioligand Binding Studies | Springer Nature Experiments.
- Article Tools?
- Radioligand Binding Studies!
Proc Natl Acad Sci. Independently evolving species in asexual bdelloid rotifers. PLoS Biol. Disentangling the morphological stasis in two rotifer species of the Brachionus plicatilis species complex. Comparative binding of antibody to a mate recognition pheromone on female Brachionus plicatilis and Brachionus rotundiformis Rotifera. Basic local alignment search tool. J Mol Biol. Clustal W: improving sensiticity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice.
Nucleic Acids Res. Guex N, Peitsch MC. Comparative protein structure modeling of genes and genomes. Annu Rev Biophys Biomol Struct. Trott O, Olson AJ. Software news and update AutoDock Vina: improving the speed and accuracy of docking with a New scoring function, efficient optimization, and multithreading.
- Kinetic analysis of estrogen receptor/ligand interactions | PNAS.
- ER-alpha Transcription Factor Activity Assay.
- Exploration of Dimensions of Estrogen Potency;
- depending on technology essay.
J Comput Chem. Collapse and recovery of green fluorescent protein chromophore emission through topological effects. Acc Chem Res.
Fluorescence response profiling for small molecule sensors utilizing the green fluorescent protein chromophore and its derivatives. ACS Comb Sci. Methods Mol Biol. Methods Enzymol. Chemical complementation: small-molecule-based genetic selection in yeast. Creation and discovery of ligand-receptor pairs for transcriptional control with small molecules. BAPJA: a yeast two-hybrid strain for both positive and negative genetic selection. J Microbiol Methods. Download references. We would like to thank the three anonymous reviewers and the editor Dr. Becks for very helpful suggestions on our paper.
Estrogen Receptor (ER) Binding Assays Using the CERI Human Recombinant ERα
We also thank Lelani Barry and Samantha Dean for their technical assistance. BLJ carried out rotifer culturing, genetic selection assays, homology modeling, completed the lifespan and reproductive assays and drafted the manuscript. CLW synthesized the fluorescent synthetic ligands and completed the docking simulations. All authors read and approved the final manuscript.
Correspondence to Brande L. Conserved amino acids are green. Similar amino acids are cyan. Amino acids that directly interact with the estradiol ligand are boxed rotifer, red; human; blue. These amino acids are highly conserved as well. XLS kb. These compounds were chosen because previous work verified their binding to the human ER LBD in yeast chemical complementation assays.
These compounds fluoresce upon binding to their target [ 35 ]. Estradiol is the natural ligand for the human ER and does not fluoresce.